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KMID : 1156220160420040266
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Journal of Environmental Health Sciences
2016 Volume.42 No. 4 p.266 ~ p.273
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Virucidal Efficacy against Avian Influenza Virus of a Disinfectant Spray Containing Grapefruit Seed Extracts, Citric Acid, Malic Acid and Benzalkonium Chloride
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Cha Chun-Nam
Park Eun-Kee
Jung Ji-Youn
Yoo Chang-Yeul
Kim Suk
Lee Hu-Jang
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Abstract
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Objectives: This study evaluated the virucidal efficacy against avian influenza virus (AIV) of a disinfectant spray containing 0.25% grapefruit seed extract, 0.2% citric acid, 0.0625% malic acid and 0.0125% benzalkonium chloride.
Methods: The disinfectant spray was diluted several times with hard water (HW) and organic matter (OM). Two point five mL of each diluent was added into each test tube, and 2.5 mL of AIV suspension was inserted into each test tube. After 30 minutes of virus-disinfectant contact reaction at , 2.5 mL of 10% inactivated fetal bovine serum was added into each test tube to neutralize the sanitizer efficacy. The neutralized solutions were serial 10-fold dilutions with phosphate buffer solution, and 0.2 mL of the diluents was injected into the allantoic cavity of five ten-day-old-chickens per dilution time. After incubation of the embryos for five days, the viability of the AIV was examined by hemagglutination titer. The valid dilution of the disinfectant spray was estimated according to the dilution time that the virus titer was inactivated more than 50% egg-infective dose (EID50)/mL compared with pathogen control.
Results: In HW and OM conditions, the valid dilutions of the disinfectant spray against AIV were seven- and three-fold dilutions, respectively. The AIV titer of the pathogen control was more than 6.1 log10EID50/mL, and there was no embryonic toxicity.
Conclusion: The present study showed that this disinfectant spray has effective virucidal activity against AIV.
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KEYWORD
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Avian influenza virus, benzalkonium chloride, citric acid, disinfectant spray, grapefruit seed extracts
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